2024.08.31論文

High-Resolution 3D Imaging of the Mammary Gland Using CUBIC Techniques

A recent study led by Hinck and colleagues investigated the molecular mechanisms of alveologenesis and lactogenesis in the mammary gland. They discovered that DNA damage due to replication stress during pregnancy activates the DNA damage response, affecting cell proliferation and differentiation in the mammary gland. The study identifies a mechanism involving the DNA damage response kinase WEE1 and suggests potential non-hormonal methods to enhance milk production.

 

 The researchers employed CUBIC-L/R techniques combined with immunostaining on 100 µm thick sections for confocal observation using the Airyscan system. This high-resolution imaging data showed the expression of DNA damage response molecules, such as γH2AX and pATR. The imaging supported the hypothesis that physiological DNA damage occurs in the mammary gland during pregnancy and that this damage increases endoreplication and milk production. 

 

【Reference】Physiological DNA damage promotes functional endoreplication of mammary gland alveolar cells during lactation. Molinuevo R. et al. Nature Communications, 15:3288, 2024. 

https://doi.org/10.1038/s41467-024-47668-9

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